Western blot analysis detected ASPH immunoreactivity in CS1 (Grade 3 conventional chondrosarcoma), CDS11 (Grade 2 conventional chondrosarcoma), CDS17 (de-differentiated chondrosarcoma), and CYZ/PNET2 neuroblastoma cells (positive control) (Figure 3A). The gene discussed is ASPH; the disease is chondrosarcoma.