This cellular analysis technique was further used to determine the percentage of cells expressing HIF-1α and HIF-2α in the primary HB cultures (Figure 3a,b), compared to those present in primary cultures derived from other brain tumors (astrocytoma, gliosarcoma, and glioblastoma), as well as in the SW48 (colorectal adenocarcinoma cell line) and ARPE-19 (immortalized human retinal pigment epithelium) cell lines. This evidence concerns the gene EPAS1 and gliosarcoma.