MAMDC2 and colorectal carcinoma: However, when RKO cells overexpressing MAMDC2 were cocultured with CAFs for 48 h, the expression of MYLK in CAFs increased significantly, indicating that the high expression of MAMDC2 in CRC cells may induce the upregulation of MYLK in CAFs through paracrine mechanisms (Figure 7D), and a similar trend was observed in HCT116 (Figure S2).