qRT-PCR analysis also revealed low or absent expression of typical prostate differentiation markers in 22Rv1-CR-1 cells, such as FOLH1 (PSMA), KLK3, ACP3 (prostatic acid phosphatases), and STEAP1, in contrast to that found in other PCa cell lines (Figure 2B). Here, FOLH1 is linked to posterior cortical atrophy.