This is related to the result observed by standard gating (Figure 2E), where we observed a significant increase only in the CXCR5+ subset of non-Treg, but not CXCR5− non-Treg, CD15s+ aTreg, or CXCR5+ nTreg in CLL patients compared to HC. This evidence concerns the gene CXCR5 and B-cell chronic lymphocytic leukemia.