To further confirm the inhibition of four mRNAs and the overactivation of related cancer miRNAs, human healthy liver cells (THLE-3) and four HCC cell lines (HepG2, HCCLM3, PLC, and Huh-7) were employed to evaluate the expression levels of the aforementioned mRNAs and related miRNAs. The gene discussed is HSPG2; the disease is hepatocellular carcinoma.