Among the 149 HNSCC cell lines listed in the Handbook of p53 Mutations in Cancer Cell Lines (http://p53.free.fr/Database/Cancer_cell_lines/HB_cell_lines.html, accessed on 14 April 2025), 7% (10 out of 149) were found to harbor two p53 mutations. This evidence concerns the gene TP53 and head and neck squamous cell carcinoma.