-Male Wistar rats weighing 180–220 g and aged 5–6 weeks.-Consumption of HFD (carbohydrate: 17%; protein: 25%; fat: 58%; lipid source: (not disclosed) for 28 days.-Intraperitoneal glucose tolerance test and intraperitoneal insulin tolerance test.-Administration of STZ (35 mg/kg i.p., single dose) in rats with insulin resistance.-Confirmation of DM by fasting glycemia 3 days after STZ: DM if glycemia ≥ 250 mg/dL.-Intervention for 56 days. This evidence concerns the gene INS and Insulin resistance.