Additionally, building on the involvement of the AKT pathway and cell cycle from our previous enrichment analysis, we tested the marker proteins of this pathway; the expressions of pAKT and Cyclin D1 were significantly decreased, suggesting that knockdown of NUDT5 suppressed tumor cell proliferation in vivo potentially through the deactivation of the AKT pathway (Figure 4F). Here, CCND1 is linked to neoplasm.