The results showed that serum concentrations of CXCL10, IFN-γ, TNF-α, and IL-10 were significantly elevated post-vaccination, accompanied by an increase in activated CD8+ T cells in peripheral blood, suggesting that this neoadjuvant vaccination can induce immune responses against GBM6-AD in these WHO grade 2 glioma patients [101]. This evidence concerns the gene CD8A and glioma.