Among the methods of inducing hyperandrogenic PCOS in animal models, androgenic steroids (DHEA, DHT and T) and letrozole (aromatase inhibitor) are the most frequently used drugs to recapitulate both reproductive and metabolic phenotypes, including elevated LH level, anovulation, increased body fat, and insulin resistance [28]. Here, PLOD1 is linked to polycystic ovary syndrome.