First, we used ThT staining to observe intracellular protein aggregates in several breast cancer cell lines: MDA-MB-231 (containing p53 R280K mutation), MDA-MB-468 (containing p53 R273H mutation), T47D (containing p53 L194F mutation), and MCF7 (containing wild-type p53). This evidence concerns the gene TP53 and breast carcinoma.