Impaired interactions between bacteria and their surrounding epithelium are responsible for bacterial infections.34–36 To assess the impact of Fn on the host cells in the asymmetric coculture system, we examined the changes in the morphology of the TIGK monolayer directly in the Transwell after coculture with three different Fn model strains (Fn 23726, Fn 25586, and Fn 10953) or the medium control for 24 h. Here, FN1 is linked to bacterial infectious disease.