We optionally performed the analysis adjusted for variability in tumor cell percentage across the tumor cuts (range: 60–100%) and noted lipases, notably, Acyl-protein thioesterase 1 (LYPLA1) and Fatty-acid amide hydrolase 2 (FAAH2), that manifested significant dysregulations across LUAD subtypes (Supplementary Fig. 6c). This evidence concerns the gene LYPLA1 and neoplasm.