To determine whether MLKL expression is responsible for CO-induced neuronal protection in a neurotoxin MPTP-induced mouse model of PD, Mlkl+/+ and Mlkl−/− mice were injected daily with MPTP for seven consecutive days and simultaneously received inhaled CO gas or Nec-1 injections for 13 days. Here, PCSK1 is linked to Parkinson disease.