And the marker genes, including midkine (MDK) and tissue inhibitor matrix metalloproteinase 1 (TIMP1), which represent the process of metabolism regulation, are overexpressed, serving as potential biomarkers.42,43 Through high-throughput multiomics analysis, we then found that hypomethylation and high expression of PRAME can promote the progression of early-stage lung cancer by regulating epithelial-to-mesenchymal transition (EMT)-related genes.44 Furthermore, the tumor immune microenvironment (TIME) forms the “soil” for lung cancer development. Here, MDK is linked to lung cancer.