Our experimental results showed that knockdown of SF3A3 significantly reduced the proliferation and migration, suppressed PI3K/AKT signaling pathway activity, and downregulated EMT marker expression in HCC cell, which indicated that silencing SF3A3 could affect the PI3K/AKT signaling pathway and inhibit EMT in HCC. This evidence concerns the gene AKT1 and hepatocellular carcinoma.