In addition, despite resistance to obesity in Cnot4 Het mice, serum levels of triglyceride and total cholesterol in Cnot4 Het mice were comparable to those in WT mice (Fig 3C), and glucose tolerance test indicated that glucose tolerance was not improved in Cnot4 Het mice (Fig 3D). This evidence concerns the gene CNOT4 and obesity due to melanocortin 4 receptor deficiency.