However, immunofluorescence analysis showed a decrease in LDLr expression in infected KCs harboring LD-amastigotes as compared to adjacent uninfected KCs, regardless of infection type (Figure 4A), along with no significant changes in total LDLr expression between uninfected, LD-S, and LD-R-infected-KCs, as observed from western blot (Figure 4—figure supplement 1A). This evidence concerns the gene TBCE and infection.