Infecting LDLr-KD-PEC with LD-S or LD-R resulted in no significant changes in intracellular amastigote numbers for both infection types as compared to scrambled RNA control (Figure 4—figure supplement 1C) confirming that although LD-R relies on increased LDL-influx for heightened proliferation, this influx is LDLr independent. This evidence concerns the gene LDLR and infection.