Finally, siRNA-mediated KD of NPC-1 in PEC (due to failure of successful KD generation in KCs), resulted in significant increase in amastigote load in response to LD-R-infection, while no such change in amastigote load was observed for LD-S-infected NPC-1-KD-PECs (Figure 6Gi–iv). This evidence concerns the gene NPC1 and infection.