Therefore, to explore new TNBC drug leads from medicinal plants, in this study, Leonotis ocymifolia was investigated and its constituents evaluated for cytotoxic activity against triple-negative breast cancer (HCC70), hormone receptor-positive breast cancer (MCF-7), and non-tumorigenic mammary epithelial cell lines (MCF-12A). Here, NR4A1 is linked to triple-negative breast carcinoma.