This included patients with non-NF1 pilocytic astrocytoma with KIAA1549::BRAF or BRAF p.V600E mutation (stratum 1), NF1-associated pLGGs (stratum 3) and those with recurrent/progressive non-NF1 optic pathway and hypothalamic pLGGs (stratum 4) [7,8]. This evidence concerns the gene NF1 and pilocytic astrocytoma.