For example, the proximity score of CD8+FoxP3+PD-1+ cells was higher in male tissues than in female tissues; patients who smoked more showed a higher proximity score of CD8+FoxP3+PD-1+ subsets; the proximity score of CD8+FoxP3+PD-L1− cells was lower in adenocarcinoma than in non-adenocarcinoma tissues: and, comparing tumors of stage IIIB/IIIC to those of stage IV, the proximity score of CD8+FoxP3+PD-1− cells was higher (Figure S6). The gene discussed is CD274; the disease is adenocarcinoma.