TLR4 and infection: Combined with the trend that the relative expression of miR-8 increased first and then decreased after AcMNPV-EGFP infected in A. chinensis, the above studies showed that miR-8 inhibited the proliferation of AcMNPV-EGFP by downregulating the expression of ie1 and vp39 genes in the early stage of AcMNPV-EGFP infection and then increased the expression of Defensin by upregulating the expression of Toll family to resist AcMNPV-EGFP infection.