Therefore, we speculate that miR-8 targets the genes of AcMNPV-EGFP at the early stage of AcMNPV-EGFP infection to inhibit the virus proliferation; at the same time, miR-8 inhibits the proliferation of AcMNPV-EGFP by regulating the expression of AMPs through Toll signaling pathway at the late stage of infection (Fig. 1C). The gene discussed is TLR4; the disease is infection.