In this study, various breast cancer cell lines and endometrial cells were used to analyze different expression levels of multiple target antigens (EGFR, FOLR1, HER2, EpCAM, NG2, ANK3 and CTNNB1) through flow cytometry and fluorescence microscopy (Figure 3, Figure 4 and Figure S2, Table 4 and Table S1). The gene discussed is EGFR; the disease is breast cancer.