Regarding the proximity of cells, we observed that the Treg/PD1-CD8+ T cell distance ratio from cancer cells, and that of Treg/total CD8+ T cells from cancer cells or macrophages, was significantly lower in the recur group than in the non-recur group (0.51-fold vs. 0.53-fold, respectively), which means that Treg cells are closer to cancer cells or macrophages than CD8+ T cells, and suggests that Treg cells in the recur group are more effective at suppressing the immune response than PD1-CD8+ or total CD8+ cytotoxic T cells are at killing cancer cells. This evidence concerns the gene CD8A and cancer.