To further validate the functional impact of GUSBP11 expression in CRC cells, si-GUSBP11 as well as a control group si-NC were transfected into the HCT116 cell line and confirmed the reduced expression of GUSBP11 and the success of transfection by qRT-PCR (Figure 3A). The gene discussed is GUSBP11; the disease is colorectal carcinoma.