PPARGC1A and melanoma: After melanoma implantation, the mice were intramuscularly injected with lenti-EC PGC1α–GFP, which was specifically expressed in the muscle endothelium as confirmed by the colocalization of IB4 and GFP (Extended Data Fig. 9e–h) and increase in PGC1α mRNA levels (Extended Data Fig. 9i).