Given the co-expression and co-localization of NRP-1 and c-Met in hepatocytes, we further investigated their interaction. Immunofluorescence analysis confirmed their colocalization on the cell membrane of both primary mouse hepatocytes and Huh7 cells (Fig. 4a–b), suggesting that NRP-1 may promote liver fibrosis as a c-Met co-receptor. Co-immunoprecipitation (Co-IP) assays demonstrated NRP-1 and c-Met binding in hepatocytes to confirm this interaction. Here, NRP1 is linked to Hepatic fibrosis.