DF2 has been found to be significantly upregulated in ischemic stroke (IS) patients and showed significant association with IS by univariate logistic regression analysis.39DF2 might accelerate this process by regulating matrilin-3.40 Additionally, DF2 may be involved in the abnormal degradation of SIRT6 and Nrf2 caused by a lower level of FTO in IS models.41,42DF2 has also been reported to be involved in Parkinson’s disease, dementia, amyotrophic lateral sclerosis, and inflammatory pain.43-47 However, the functions of DF2 in human neuroectodermal development remain largely unknown. This evidence concerns the gene SIRT6 and amyotrophic lateral sclerosis.