This study developed a predictive nomogram for early neurological deterioration (END) in acute ischemic stroke (AIS) using serum biomarkers tissue plasminogen activator–inhibitor complex (t‐PAIC) and plasmin ‐α2 plasmin inhibitor complex (PIC), age, National Institutes of Health Stroke Scale (NIHSS) score, lymphocyte (lymph), and platelet (PLT). The gene discussed is PLG; the disease is stroke disorder.