To determine whether CD19/NKG2DL tandem CAR T-cells can effectively target primary B-ALL tumor cells, we incubated T-cells expressing the tandem with the CD8 short hinge, alongside mock and single CAR T-cells, with CD19+ bone marrow specimen from two B-ALL patients (Immunophenotyping available in Table 1). Here, CD19 is linked to acute lymphoblastic leukemia.