To reduce the possibility of not confirming a true polio case, ≥80% of AFP case-patients should have adequate stool samples, defined as two stool samples of sufficient quantity for testing, collected at least 24 h apart and within 14 days of paralysis onset (timeliness), and arriving to an accredited poliovirus laboratory in good condition (quality) (2, 4). This evidence concerns the gene AFP and Paralysis.