To investigate whether HK2 is involved in the SPARC‐mediated 5‐FU chemoresistance in CRC, we performed colony formation and CCK‐8 assays and found that the knockdown of HK2 abolished the effects of SPARC overexpression on the proliferative capacity and viability of RKO and HCT116 cells under 5‐FU treatment (Figure 4A,B). Here, HK2 is linked to colorectal carcinoma.