Similarly, survival analyses showed that the presence of CD4 + or CD8 + T cell subsets in close proximity and clustering around tumor cells were both associated with a good prognosis in patients with ESCC, and whether these TILs expressed LAG3 in fact exerted some influence, suggesting that LAG3 exhibits a close association with immune activation in tissues treated with CCRT, even though LAG3-positive TILs in pre-treatment tissues seem to exhibit a trend towards closer resemblance to immunosuppression. This evidence concerns the gene CD4 and esophageal squamous cell carcinoma.