To investigate if the prevention of dysbiosis by I3C during colitis results in microbiota that promotes AhR or BD‐1 expression, we collected the colonic content microbiota from control, anti‐CD40‐induced colitis, and anti‐CD40+I3C treated mice, and cocultured with MC38 and Caco2 cells for 24 h. This evidence concerns the gene DEFB1 and colitis.