ITGB3 and osteosarcoma: Based on siRNA transfection, short-term ITGB3-knockdown (ITGB3-KD) and ITGB3-negative control (ITGB3-NC) HOS and U2OS osteosarcoma cells were constructed, and the effectiveness of ITGB3 protein knockdown was verified by western blotting (Fig. 1E, F).