Integrating the findings from the aforementioned mechanism investigations with those of a series of rescue experiments and osteogenic differentiation cultures, we have substantiated that ITGB3-KD potentiates osteogenic differentiation and exerts proapoptotic radiosensitizing effects in osteosarcoma by activating the JNK/c-JUN/RUNX2 pathway. The gene discussed is RUNX2; the disease is osteosarcoma.