It was found that the effect of PAAG stiffness on the CD8+ T cells cytotoxicity to USP8KO DU145/PC-3 cells and USP8C786A DU145/PC-3 cells was lost, indicating the vital role of USP8 and its deubiquitinating enzyme activity in the process of matrix stiffness promoting immune escape of PCa (Fig. 3K–N). Here, CD8A is linked to posterior cortical atrophy.