If we overexpressed wild-type NBR1 in PCa cells cultured in low PAAG stiffness, MHC-1 was down-regulated, and if we overexpressed K887R-NBR1 in PCa cells cultured in low PAAG stiffness, MHC-1 was further down-regulated compared with those transfected with wild-type NBR1 (Fig. S8). Here, NBR1 is linked to posterior cortical atrophy.