To examine the impact of ALS-associated pathological mutants on cellular MLOs of PFN1, we expressed EGFP-labeled wild-type or ALS-linked PFN1 mutants (A20 T, C71 G, T109 M, M114 T, E117 G, G118 V, R136 V, and Q139 L) in HEK 293 cells. The gene discussed is PFN1; the disease is amyotrophic lateral sclerosis.