Most transplanted NPCs differentiate into astrocytes and neurons within the first month, as shown in previous studies.[12] To identify whether the transplantation timing after stroke can affect the graft composition, we phenotyped human nuclei (HuNu+) positive cell grafts for pluripotent stem cell (Nanog), NPC (Pax6), mature neuronal (NeuN), and astrocytic (GFAP) markers (Figure 3D,E). This evidence concerns the gene PAX6 and stroke disorder.