Our previous sequencing data have shown that UM‐SCC1 (SCC1) and MDA686TU (Tu686) cells harbor mutations in the FAT1 gene.[11] To understand the necessary dependency of mutant FAT1 in HNSCC cells, we performed a CRISPR/Cas9‐mediated gene KO of FAT1 in SCC1 and Tu686 to create FAT1 KO cells (Figure 1F). Here, FAT1 is linked to head and neck squamous cell carcinoma.