To this end, we resorted to a small interfering RNA (siRNA) approach to knockdown the RPS19 mRNA in HCT116 cells, a colorectal cancer cell line suitable to identify alterations induced by ribosome biogenesis defects,49 study mechanisms of mRNA stability and translation regulation in ribosomal protein deficits,50 and dissect apart p53+ and p53-dependent processes using its TP53 null isogenic variant.51 This evidence concerns the gene TP53 and colorectal cancer.