Such as how radiation induces AQP4 expression changes, how AQP4 regulates downstream signaling pathways, and how AQP4 changes cell-cell tight junctions in the brain tissue, the optimal time window for AER-271 treatment and how it can be transferred to clinical applications, and whether AQP4 treatment can improve advanced cognitive impairment. This evidence concerns the gene AQP4 and Cognitive impairment.