From the initial 15, we narrowed it down to five: our analysis showed that the quantitative determination of IFN-γ, in parallel with the chemokine IP-10 and three pro-inflammatory cytokines (IL-6, IL-1α and IL-1β) could be useful in the diagnosis of brucellosis in Mediterranean Buffaloes. The gene discussed is CXCL10; the disease is brucellosis.