The UMAP plots of the downward clustering based on the regulatory activities of TFs were less discrete, and the distributions of C0 IGLC3+ Myeloma cells and C2 IGHG1+ Myeloma cells were more clearly delineated; similarly, the histological characteristics of four myeloma cell subsets were more favorable to MM. This evidence concerns the gene IGHG1 and Miyoshi myopathy.