IGHE and allergic disease: Allergic reactions are triggered by exposure to allergens, or usually harmless substances, such as dust, pollen, pet hair, etc., that induce the immune system into an immediate overactive Th2 inflammatory response by recruitment and expansion of cells, and production of cytokines, histamine, IgE, leukotrienes, etc. We measured soluble cytokines in culture supernatants by multiplex array and the production of allergy-specific products IgE, leukotriene B4, and histamine by ELISA.