Our outcomes revealed that the levels of RAS, MEKK1, ERK, IKB, and NF-kB in the AOM/DSS group were significantly upregulated in contrast to the control group (Figures 11B–F, p < 0.001, p < 0.05, p < 0.01, p < 0.0001, p < 0.001). The gene discussed is MAP3K1; the disease is infectious otitis media.