The similar amyloid plaque burden in the brain of AD and MIX mice was surprising given previous work from our group45 showed 3× more plaques in the MIX versus the AD mice; however, these differences could be due to the different AD mouse models used (in the previous study the J20 mouse, and here, APP/PS1, which has a more severe plaque load), as well as the different analysis methods. This evidence concerns the gene APP and Alzheimer disease.