6 A-D). Thus, 200 HF samples from the GSE141910 were categorized into two distinct groups, Cluster A (n = 68) and Cluster B (n = 132). PCA plot revealed distinct gene expression patterns between two clusters (Figure. 6E). Distinct m5C-related gene levels were found between two clusters. ALYREF were greatly expressed in Cluster A, while NSUN6, DNMT3A, NSUN7, NSUN3, TRDMT1, DNMT1, and DNMT3B were greatly expressed in Cluster B (Figure. 6 F). This evidence concerns the gene DNMT3B and hydrops fetalis.