We report a 4 to 6-fold enrichment of breast cancer associated EV miRNA in both HER2 and CD24 antibody labeled sample compared to isotype antibody immunolabeled samples for the BT-474 and MDA-MB-453 spike-in models (Student’s t-test p < 0.05) (Supplementary Fig. S3E, G). Here, ERBB2 is linked to breast carcinoma.