Interestingly, multiple regression analysis resulted in a negative association between an RA diagnosis and the proportion of IFNγ+CD4+ memory T cells and positive associations between a TNFi treatment and the proportions of IFNγ+CD8+ effector T cells and Th17 cells, whereas a treatment with methotrexate or leflunomide did not contribute significantly to the regression models. The gene discussed is CD4; the disease is rheumatoid arthritis.